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Excellent Phase Contrast in the μ-Slide 4 well Ph+
一個開放的四孔培養載玻片，帶有一個特殊中間板，非常適用于相差和高端的熒光顯微鏡

整個培養孔具有優秀的相差-沒有彎月面現象

一體化小室，既可用于細胞培養，也可用于顯微鏡成像

使用高分辨率顯微鏡可以通過玻璃片底部進行樣品觀察

沒有蓋玻片，不會泄露，用于快速，簡單的免疫熒光。

應用：
細胞培養以及細胞培養物的顯微鏡觀察。
轉染分析
活細胞和固定細胞的免疫熒光顯微鏡觀察
長時間段下活細胞成像

貨號	產品名稱	規格（個/盒）
80446	μ-Slide4孔Ph+腔室載玻片，ibiTreat底部處理	15
80442	μ-Slide 4孔Ph+腔室載玻片，Collagen IV底部處理	15
80443	μ-Slide 4孔Ph+腔室載玻片，Fibronectin底部處理	15
80444	μ-Slide 4孔Ph+腔室載玻片，Poly-L-Lysine底部處理	15
80445	μ-Slide 4孔Ph+腔室載玻片，Poly-D-Lysine底部處理	15
80441	μ-Slide 4孔Ph+腔室載玻片，無包被	15
80447	μ-Slide 4孔Ph+腔室載玻片，玻璃底	15

技術指標：

孔數量	4	帶蓋子高度	10.8 mm
孔尺寸大小	21.2 x 11.0 x 3.0	每孔生成面積	2.2 cm2
每孔體積	700 μl	每孔生成面積	5.9 cm2
底部ibidi 標準底部

技術特點：

開放式，兩個獨立的培養孔載玻片
每個培養孔上的中間板可以避免彎液面形成
從兩側縫隙很容易加液，無氣泡形成，
適用于高端顯微鏡的優秀光學成像特質
兼容染色，固定
最佳細胞粘附的表面Ibitreat
生物兼容性塑料生產，無膠水，不泄露

Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the μ-Slide 4 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

Cross Section Through a Well of the μ-Slide 4 well Ph+ with a Transmitted Light Beam Path.

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the μ-Slide 4 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.